Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello Fredrik,
The following link will take you to a PDF file of a short article
discussing controls for immunolabeling (Microscopy Today, November 2001).

http://www.itg.uiuc.edu/people/garsha/documents/Immunolabeling.pdf

Cheers,
Karl G.

Fredrik Swift wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello,
>
>I'm planning some immunolabelling experiments. I will use primary antibodies
>agains subunits of the Na/K-ATPase and the Na/Ca-exchanger in permeabilized
>rat cardiomyocytes and secondary antibodies coupled to an Alexa-fluorochrome
>(Mol. Probes). What would be appropriate controls in this type of
>experiment?
>- Without primary antibody?
>- Non permeabilized cells?
>- Any other good positive/negative controls?
>
>Thanks,
>
>Fredrik
>
>