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Hi Andy,
As an alternative, you might think about a chamber that encloses your
imaging system.  Pretty much all the confocal systems can be had with one to
fit most any scope.  In this case, you simply perfuse humidified 5% CO2, 95%
air through the enclosure.  One of the benefits is that the whole imaging
system is equilibrated to the same temperature, so problems associated with
a temp gradient between objective and sample, as well as focus fluctuations
over time, are minimized.
Cheers,
Carl
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-626-8469
FAX 520-621-3709
----- Original Message -----
From: "Andrew Resnick" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, March 11, 2005 12:16 PM
Subject: Q: CO2 perfusion


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Several of us are putting in a NCRR Shared Instrumentation Grant for an
> upright scope to eprform live-cell studies.  There does not appear to be a
> commercial solution for atmospheric or pH control- those systems are made
> for inverted scopes.  We have heard that gently superfusing a 5% CO2
> mixture over the top of the cells is sufficient due to the density of CO2
> with respect to air.  Is there someone who has done this?  If so, please
> let me know as I would like to include a reference to this method in the
> application.  Thanks in advance,
>
> Andy
>
> Andrew Resnick, Ph. D.
> Fellow
> Department of Physiology and Biophysics
> Case Western Reserve University
> 216-368-6899 (V)
> 216-368-4223 (F)