Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I recently built an A1 using Ludl XY stage and Z motor, all controlled
through Ludl MAC 5000 controler. We use the system for stereology. If you
don't get the upgrade, the A1 is still a very flexible stand. Because we
cover a lot of disciplines here, our A1 was built to be capable of tiling,
z-stacking, time series, transmitted light imaging, polarized light imaging,
fluorescence imaging (Ludle 10 port filter wheels) and reflected light
imaging. If you go with the motorized versions and add the Axiovision
software for control along with Zeiss cameras I think you will be quite
happy.
We have an inverted Axiovert 200M that can do limited live cell work; it
also does everything the A1 does, except it only has five fluorescent
channels (no filter wheels) and uses Axiovision software. The key to success
with the Axiovision software is to buy the Zeiss cameras also. We have a
Roper EMCCD and a Zeiss Axiocam and there are features like shading
correction that are locked out of the multidimensional acquisition setup in
for the Roper camera making it harder to work with than the Zeiss camera.
You can still do shading correction it just takes more steps.
Both A1 and the Axiovert 200M are used quite heavily and require little
maintenance. When a part does break it can be exchanged at a significantly
reduced cost through a parts exchange program.
Cheers,
Jonathan M. Ekman
Imaging Technology Group
Beckman Institute for Advanced Science and Technology University of Illinois
at Urbana-Champaign
405 N. Mathews Avenue
Urbana, IL 61801 USA
Tel: 217-244-6292
Fax: 217-244-6219
_____
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Farid Jalali
Sent: Wednesday, February 27, 2008 12:08 PM
To: [log in to unmask]
Subject: Thoughts on Axioimager line of Microscopes/ Imaging system
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello All,
I would appreciate hearing thoughts on Zeiss' line of Axioimager imaging
systems. I've just found out that an A1 (most basic, for documentation) is
going into our tiny (2 scope) facility. However, I am thinking of asking for
an upgrade to the M1, motorized and apparently suitable for live cell
imaging, 3D deconvolution microscopy, FRET even. Is anyone out there using
their Axiovision's for live cell imaging, FRET, 3D acquisition and
deconvolution?
Any and all thoughts are greatly appreciated.
Cheers
Farid
--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
Princess Margaret Hospital
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
416-581-7754 STTARR at MaRS Building
416-581-7791 STTARR Microscopy Suite
|
