Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Orin,

thanks for the information, we currently in the process of writing the 
grant for the scope and would appreciate any additional information. if 
you have any preferences after the demo's please pass that information 
along.

thanks!
Perveen

ORIN HEMMINGER wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi,
>
>We recently got a demo of the Olympus DSU spinning disk confocal system, it uses slits instead of pinholes in the disk which lets more light through and allows them to use a mercury lamp for illumination instead of a laser line like the nipkow spinning disks require.  The system may lose a little resolution compared to the Yokogawa disk with a laser line but it worked quite well and it has several advantages.  First it is cheaper and easier to use than laser based systems, and they have different disks that are optomized for different objectives so you can do confocal from 100x all the way down to 10x, where as pin hole systems are optomized for 100 or 80x and if you go lower than that you'll lose confocallity.  Plus if you want a different excitation wavelength you just have to get a different filter, not a whole new laser line.  I'm in the process of still getting demos of other high speed systems, but this one worked quite well.  You can get 35 frames per second at full f
>rame (512x512) if you use the EMCCD camera that is avaliable.  The full turn key system runs just under $200K which is significantly cheaper that the Yokogawa/laser based systems, but I haven't demod those yet so maybe they have significanly better resolution that may be worth it?  Regardless, if your worried about the laser lines, the Olympus DSU would be worth looking into.
>
>Best of luck,
>
>-Orin Hemminger
>ChBE Graduate Student
>The Ohio State University
>[log in to unmask]
>
>----- Original Message -----
>From: Perveen Biln <[log in to unmask]>
>Date: Friday, September 23, 2005 1:31 pm
>Subject: purchasing an new scope
>
>  
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Hello,
>>
>>We are looking to purchase a new microscope for live cell imaging 
>>and I 
>>was hoping to get some feedback regarding the general advantages 
>>and 
>>disadvantages of the different options.  Currently, we can choose 
>>between a confocal such (as the zeiss meta), a spinning disk or a 
>>two-photon.  One the main concerns against purchasing a confocal 
>>is that 
>>many of the researchers would like use UV light, but are concerned 
>>about 
>>the cost of purchasing and maintaining a UV laser.  Comments 
>>regarding 
>>the most cost-effective scope and on the advantages / 
>>disadvantages of a 
>>UV laser would be appreciated.
>>
>>thank you!
>>
>>-- 
>>Perveen Biln, M.Sc.
>>Microscopy and Imaging Consultant
>>Institute for Health Research and Education
>>Phone:	604.268.6807
>>Email:	[log in to unmask]
>>
>>    
>>

-- 
Perveen Biln, M.Sc.
Microscopy and Imaging Consultant
Institute for Health Research and Education
Phone:	604.268.6807
Email:	[log in to unmask]