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Date: | Wed, 17 Jun 1998 09:05:07 +1000 |
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>I will require some non-fading fluorescent standards (red &
>green) that I can use to set up the microscope before testing my yeast
>samples. I have been told that some plastics (some kinds used to make
>cheap toys) can work very well. I don't know how true this is or
>what plastics would be good. I would really appreciate any
>suggestions anyone might have on appropriate standards that I could
>use.
Your local manufacturer of plastic signs should be able to give you
a few offcuts. There is a greenish-yellow one and a red one both
of which should be suitable for your needs (they also work fine as
standards for FITC and rhodamine).
However, if it in only the _relativity_ between green and red stains
you need to measure, standards may be unnecessary - you can just take the
ratio of the two intensities.
Guy
Dr. Guy Cox, | ooOOOOOOoo
E.M. Unit, F09 | # oOOOO | | OOOOo #
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