CONFOCALMICROSCOPY Archives

June 1998

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 17 Jun 1998 09:05:07 +1000
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>I will require some non-fading fluorescent standards (red &
>green) that I can use to set up the microscope before testing my yeast
>samples.  I have been told that some plastics (some kinds used to make
>cheap toys) can work very well.  I don't know how true this is or
>what plastics would be good.  I would really appreciate any
>suggestions anyone might have on appropriate standards that I could
>use.

Your local manufacturer of plastic signs should be able to give you
a few offcuts.  There is a greenish-yellow one and a red one both
of which should be suitable for your needs (they also work fine as
standards for FITC and rhodamine).

However, if it in only the _relativity_ between green and red stains
you need to measure, standards may be unnecessary - you can just take the
ratio of the two intensities.

                                                        Guy

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