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We've had a slew of 'answers' to this few of which have addressed
Sarah's actual question!

Believe it or not if these are Bio-Rad files and you have somewhere
(in your basement?) and old MRC600 or 1000 running COMOS/MPL it's
dead easy to do this in an MPL macro.  It's easiest if the individual
images at each plane tile perfectly but even if they overlap it can
be done with little effort since MPL supports 'putting' a picture at
arbitrary coordinates - you'd just have to work out the coordinates
for overlapping the component stacks.  The macro will then run through
the original multiple stacks and output a composite one.

Limitation - the final image size at each plane can't exceed what the
system eill handle (768*512 on MRC 500/600, I think 1024*1024 on an
MRC 1000).  So you may have to scale each image - but of course MPL will
do that for you too.  It would be pretty hard to handle the resultant
output stack if each plane got larger than that anyway.

I'll cheerfully write you a macro if you want to try it that way.

My guess would be that any good image analysis program (Image J,
KS400) which has macro programming could also do it but it's a breeze
in MPL.

                                                        Guy



 Hi- Does  anyone know of a program that will create a 3D montage of image
stacks?   The only thing I can think of right now is for the user to create
montages in  photoshop, save each montage slice as a tiff and then open
them as a stack in  some 3D rendering program.  Any ideas about an easier
way to do  this? Thanks- Sarah
>
>-------------------------------------------------------------------------------
>--------
>Sarah Locknar, Ph.D.
>Director, Neuroscience COBRE Imaging / Physiology Core
>College of Medicine, University of Vermont
>E015 Given Building
>89  Beaumont Ave.
>Burlington, VT 05405
>802-656-0413
>802-656-8704 (fax)
>-------------------------------------------------------------------------------
>--------
>
>


Assoc. Prof. Guy Cox,                 ooOOOOOOoo
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