Could I just chip in and say that in one way, it would be
     misleading to retain a 2D scale on a 3d data set projected into 2D.
     This is because on a projection, making an xy measurement between
     features which are also separated in z would simply be a mistake!

     Anna Smallcombe PhD
     Senior Applications Specialist
     Bio-Rad Microscopy Division


______________________________ Reply Separator _________________________________
Subject: Re: Saving Bio-Rad images
Author:  Confocal Microscopy List <[log in to unmask]> at
Internet
Date:    28-7-1999 11:25 am


DOUG : I second Rob Palmer's comments. The color-merged Bio-Rad files
will be difficult to work with in other programs. For example, LUT's
often differ between companies.

    Although you did not ask about it, I would pass on another caution
to your users. In the Bio-Rad Lasersharp 1024 software, if you select
"Project" which is the command to superimpose a group of successive
Z-images of the same field, the scale information is lost. That means
that if you Project the images without doing anything special and only
save the projection, you will not be able to do any measurements later
on, and cannot add a scale bar. One way to avoid this is to have your
users recall the Z-series stack and select Scale Bar from the Select
list [I am not at the CLSM so I hope my memory is correct; if not, try
the Measurement list] so that a scale bar appears on one of the images
of the Z-series stack and then re-save. Then when you project your
images, the scale bar appears and will save with your image. However,
note that once you do this, the scale bar is permanently visible in your
image.

    If everyone knows about the above already, my apologies.

ANDREA ELBERGER

--
Dr. Andrea J. Elberger
Professor, Anatomy and Neurobiology
Director, Confocal Laser Scanning Microscope Facility
The University of Tennessee, Memphis
855 Monroe Avenue
Memphis, TN  38163  U.S.A.
tel: 901-448-4101
FAX: 901-448-7193
<mailto:[log in to unmask]>