Dear All,
I would like to get the lists input on this too. I have an image that best
conveys the information by projecting the 3-D set into 2D. As a scale bar
seems inadequate, what is the best way to scale this image?
Also, we just purchased Bitplane and are very happy with it, I would like
to recommend this software package to the list. No commercial interest
either!
Thanks,
-Guy
**************************************
Guy Cook
President
Bacterin Inc.
P.O. Box 6743
Bozeman, MT 59771-6743
(406) 582-8184
[log in to unmask]
http://www.bacterin.com
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> From: Anna Smallcombe <[log in to unmask]>
> To: [log in to unmask]
> Subject: Re: Saving Bio-Rad images
> Date: Thursday, July 29, 1999 5:09 PM
>
> Could I just chip in and say that in one way, it would be
> misleading to retain a 2D scale on a 3d data set projected into 2D.
> This is because on a projection, making an xy measurement between
> features which are also separated in z would simply be a mistake!
>
> Anna Smallcombe PhD
> Senior Applications Specialist
> Bio-Rad Microscopy Division
>
>
> ______________________________ Reply Separator
_________________________________
> Subject: Re: Saving Bio-Rad images
> Author: Confocal Microscopy List <[log in to unmask]> at
> Internet
> Date: 28-7-1999 11:25 am
>
>
> DOUG : I second Rob Palmer's comments. The color-merged Bio-Rad files
> will be difficult to work with in other programs. For example, LUT's
> often differ between companies.
>
> Although you did not ask about it, I would pass on another caution
> to your users. In the Bio-Rad Lasersharp 1024 software, if you select
> "Project" which is the command to superimpose a group of successive
> Z-images of the same field, the scale information is lost. That means
> that if you Project the images without doing anything special and only
> save the projection, you will not be able to do any measurements later
> on, and cannot add a scale bar. One way to avoid this is to have your
> users recall the Z-series stack and select Scale Bar from the Select
> list [I am not at the CLSM so I hope my memory is correct; if not, try
> the Measurement list] so that a scale bar appears on one of the images
> of the Z-series stack and then re-save. Then when you project your
> images, the scale bar appears and will save with your image. However,
> note that once you do this, the scale bar is permanently visible in your
> image.
>
> If everyone knows about the above already, my apologies.
>
> ANDREA ELBERGER
>
> --
> Dr. Andrea J. Elberger
> Professor, Anatomy and Neurobiology
> Director, Confocal Laser Scanning Microscope Facility
> The University of Tennessee, Memphis
> 855 Monroe Avenue
> Memphis, TN 38163 U.S.A.
> tel: 901-448-4101
> FAX: 901-448-7193
> <mailto:[log in to unmask]>
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