*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Confocalists,

I am in desperate need for a new fuorophores for my multicolor FISH-
immunofluorescence experiments. I am  using at the moment DAPI, Alxea488, Cy3, 
Texas Red and Cy5. I use for imaging Zeiss microscope LSM510meta.
I am thinking about purchasing antibodies conjugated with Alexa 680 or Alexa 
700. According to olipmpus fluorophore tutorial web page my 633 nm laser should 
be able to excite both although its quite far away from excitation maximum.
Has anybody used successfully any of these fluorophores with LSM510 meta? 
Is it easy to separate them from Cy5 using meta detector?
Molecular mass of of these fluorophores is relatively high (1.2-1.4 kDa) compared 
with the fluorophores which I am using right now, will it have effect on the 
antibody penetration ?

Kind regards,

Michal Gdula
PhD student
Bradford University
Uk