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Dear Shiv,
You may want to consider heating the inflow by wrapping the tubing  
around a heat source placed near the chamber.  We use a length of  
heat tape epoxied to a piece of metal rod.

Regards,
Glen
On May 24, 2006, at 12:29 PM, Sivaguru, Mayandi wrote:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi- 
> bin/wa?S1=confocal
> Hello all, we would like to buy a peristaltic pump for our POC-R  
> closed/open system heated stage insert. We found the POC-R closed  
> system is requiring flow rate of (closed chamber): 0.1-0.25ml/hr.  
> Is this speed is absolutely required? We are wondering with this  
> speed how can one supply different test solutions to sample in a  
> reasonable time? With this speed the test solution may stay in the  
> tube for a longer time and may get cold before it reaches the  
> heated chamber as we do not heat the transfer tubes to 37 degrees.  
> On the other hand, the pumps from different sources Bioptechs,  
> Ismatec, Instechlabs, Watson marlow sci-q are priced significantly  
> different.
>
>
>
> I would really appreciate your comments, if you use these  
> accessories routinely for live cell imaging and if so what are the  
> pros and cons of the pumps from different manufacturers, ease of  
> use, precision and what would be the flow rates normally used for  
> closed (adherent cells) or open system (tissue slices) heated stages.
>
>
>
> Thanking you in advance for your insights.
>
> Shiv
>
>
>
>
>
> Mayandi Sivaguru, PhD
>
> Associate Director
>
> Molecular Cytology Core
>
> Research Core Facilities
>
> 120 Christopher S. Bond Life Sciences Center
>
> University of Missouri
>
> Columbia, MO 65211
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>
>
> Voice: 573-882-4895
>
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> www.biotech.missouri.edu/mcc
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