CONFOCALMICROSCOPY Archives

December 1999

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Subject:
Re: Fluorescence intensity
From:
Kees Jalink <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 23 Dec 1999 15:43:13 +0100
Content-Type:
text/plain
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text/plain (23 lines) 
Rolland,

if your microscope is of the fibre-coupled type and if , if you measure
over prolonged time(hours), the signal actually fluctuates (oscillates)
rather than steadily goes up, you are dealing with a phenomenon that has
to do with polarization effects on the dichroics/AOTF.
In that case, check the emaillist of about one month ago where we
discussed this in extenso. No cure yet.

Otherwise, pray, or refocus your research to running old fasioned gels
again.

Merry Xmas etc, Kees

--

Kees Jalink Ph.D.
The Netherlands Cancer Institute, dept. of Cell Biology H1
Plesmanlaan 121, 1066CX Amsterdam, the Netherlands
020-5121933 (tel)   / 020-5121944 (fax) / [log in to unmask] (email)
at home: Paulus Potterlaan 5, 2102CC Heemstede
0235-476047 / [log in to unmask]

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