Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We are using Fura Red (MolProbes) as a ratiometric calcium indicator for
visible light confocal imaging, blue excitation (ratio between 457 and
488 nm), the emission is above 600 nm. It's not the best indicator in
terms of quantum yield and dynamic range, but it's the only one that can
be used as a single indicator for ratiometric visible light confocal
calcium imaging. I have discribed the method in
Lohr, Cell Calcium 34, 295- , 2003.
I can send you the pdf if you like.

Best regards
Chris

Kathy Spencer wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi All!
>        Having difficulties with ligand autofluorescence in calcium
> imaging. We
> measure an auto-fluorescence peak around 460-570nm emission, and are
> trying
> Fura-2 and Rhod-2 calcium indicators. Our compound still interferes with
> the emission of these dyes. Can anyone recommend other calcium dyes we
> could try? We found that the Fluo dyes are right in the middle of our
> autofluorescence peak, but are there other dyes?
>        Thanks!
>        Kathy Spencer
>
>
>
> Kathy Spencer, Ph.D.
> Scientific Associate
> The Scripps Research Institute
> 10550 N. Torrey Pines Road
> ICND 202
> La Jolla, CA 92037
> 858-784-8437
>
>

--
Dr. Christian Lohr
Abteilung für Allgemeine Zoologie
Universität Kaiserslautern
Postfach 3049
67653 Kaiserslautern
Germany

Phone: +49-(0)631-205 3689
Fax:   +49-(0)631-205 3515
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http://www.uni-kl.de/FB-Biologie/AG-Deitmer/homepages/lohr/lohr.html