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March 2004

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Subject:
Re: calcium indicators
From:
Kathy Spencer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 18 Mar 2004 09:15:35 -0800
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Chris!
         Yes, please...can you send the PDF? I'm pretty new to calcium 
imaging, and don't know what to try next. Thanks for your reply.
         Kathy




At 09:12 AM 3/18/04 +0100, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We are using Fura Red (MolProbes) as a ratiometric calcium indicator for
>visible light confocal imaging, blue excitation (ratio between 457 and
>488 nm), the emission is above 600 nm. It's not the best indicator in
>terms of quantum yield and dynamic range, but it's the only one that can
>be used as a single indicator for ratiometric visible light confocal
>calcium imaging. I have discribed the method in
>Lohr, Cell Calcium 34, 295- , 2003.
>I can send you the pdf if you like.
>
>Best regards
>Chris
>
>Kathy Spencer wrote:
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Hi All!
>>        Having difficulties with ligand autofluorescence in calcium
>>imaging. We
>>measure an auto-fluorescence peak around 460-570nm emission, and are
>>trying
>>Fura-2 and Rhod-2 calcium indicators. Our compound still interferes with
>>the emission of these dyes. Can anyone recommend other calcium dyes we
>>could try? We found that the Fluo dyes are right in the middle of our
>>autofluorescence peak, but are there other dyes?
>>        Thanks!
>>        Kathy Spencer
>>
>>
>>
>>Kathy Spencer, Ph.D.
>>Scientific Associate
>>The Scripps Research Institute
>>10550 N. Torrey Pines Road
>>ICND 202
>>La Jolla, CA 92037
>>858-784-8437
>>
>
>--
>Dr. Christian Lohr
>Abteilung für Allgemeine Zoologie
>Universität Kaiserslautern
>Postfach 3049
>67653 Kaiserslautern
>Germany
>
>Phone: +49-(0)631-205 3689
>Fax:   +49-(0)631-205 3515
>email: [log in to unmask]
>http://www.uni-kl.de/FB-Biologie/AG-Deitmer/homepages/lohr/lohr.html

Kathy Spencer, Ph.D.
Scientific Associate
The Scripps Research Institute
10550 N. Torrey Pines Road
ICND 202
La Jolla, CA 92037
858-784-8437

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