*****
To join, leave or search the confocal microscopy listserv, go to:
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*****
As Guy correctly mentioned, the OPS technology is unique to Coherent and uses a diode-pumped semiconductor chip to generate infrared radiation that is then intra-cavity doubled or tripled to produce CW visible/UV light. This technology is flexible in wavelength and highly scalable in power. For example, Coherent offers OPS devices with wavelengths in the range 460-589 nm and power levels ranging from milliwatts to 10 watts. Frequency-tripled versions operate at 355 nm. A number of products using OPS technology are on display this week at Neuroscience. Details of these products and the OPS technology can be found at http://www.coherent.com/products/?771/Optically-Pumped-Semiconductor-Laser-OPSL-Technology
Best regards,
Marco Arrigoni
Director of Marketing
Scientific Market Segment
Coherent, Inc.
408-764-4661
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Guy Cox
Sent: Friday, November 11, 2011 4:45 AM
To: [log in to unmask]
Subject: Re: FRET in the time of DPSS
*****
To join, leave or search the confocal microscopy listserv, go to:
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*****
OK, this is quite a confusing area (and the manufacturers do not always
make things as clear as they could).
A simple diode laser is a pure semiconductor device. The laser cavity
is contained within a single chip (basically an LED with mirror layers
above and below it). Wavelengths currently available are from near UV
(~365nm) through violet to blue (~470nm), and then down in the red from
~640nm. Because the laser cavity is so small the wavelength is not very
precise, which can be a problem for AOD based devices, though it is not
likely to be a biological problem. Your 405 and 440 nm lasers are most
likely simple diodes, as well and your 647nm. If you have a red laser
pointer this will be a simple diode.
OPS (optically pumped semiconductor) lasers are semiconductor devices
which are not excited by electric current but by input from a diode
laser. They can access wavelengths which direct diodes cannot. I think
it is fair to say that this has been very much Coherent technology.
488 - 515 lasers, which are tricky from other sources, are within this
range.
DPSS are solid state crystal lasers pumped by red diode lasers.
Neodymium Yttrium Aluminium Garnet (NdYAG) is the classic, working at
1064nm. For our purposes we are mostly interested in lasers which are
frequency doubled by second harmonic generating crystals and the classic
is therefore 532nm - the wavelength of your green laser pointer and the
laser which pumps your TiS laser for multiphoton microscopy. Ytterbium
lasers give 1030 nm (and hence 515nm when doubled). 561 nm is another
option, and I believe it comes from a secondary line from an Nd YAG.
Correction from laser companies welcome, but I hope this helps.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Craig Brideau
Sent: Friday, 11 November 2011 12:05 PM
To: [log in to unmask]
Subject: Re: FRET in the time of DPSS
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Direct diode means that the laser has a single element. It tends to be
the
simplest type of laser; basically it is a big laser pointer.
Diode-pumped
solid state use an infrared diode (typically) which pumps some secondary
medium that gives you your desired wavelength. They are more complex
than
direct diode since you now have two separate objects that have to be
reliably optically coupled. Until recently direct diode lasers have
only
been capable of near-IR, Red, and Violet or Blue lines, but a recent
breakthrough in laser diode chemistry has allowed them to work at around
520nm.
Here's an old article from 2009 mentioning one of the first green direct
diode lasers:
http://displaydaily.com/2009/07/21/green-laser-diode-demonstrated-a-brea
kthrough/
The current problem is pushing them to longer wavelengths. Projector
companies want to get them out to 530, 540nm for making displays and
video
projectors:
http://www.qmed.com/mpmn/article/suppliers-chip-based-diode-makes-waves-
green-laser-world
But 'shorter' direct diode green lasers in the 520nm or less range are
fairly available right now.
Craig
On Thu, Nov 10, 2011 at 5:09 PM, Jen Clarke
<[log in to unmask]
> wrote:
> Hi all
>
> This discussion is very timely for our facility, as we are looking to
> purchase a solide state laser in the ~500-520nm range as soon as
possible
>
> I am insufficiently familiar with the pros and cons of choosing a DPSS
> versus a OPS or a direct-diode laser
>
> From the discussion so far it sounds like the choices for a solid
state
> laser for imaging YFP are 515nm DPSS from Spectra-Physics, a 505nm
Sapphire
> OPS, a 514nm Sapphire OPS or a 520 direct diode.
>
> Guy - I presume by "direct diode" you mean a diode pumped solid state,
> which I understand would be not as good as a DPSS (please correct me
if I
> am wrong)
>
> I dont have any idea how an OPS compares to a DPSS laser
>
> Any advice as to which of these options might be "best" as an add on
to a
> new Olympus FV1000 for the primary purpose of imaging and bleaching
YFP in
> the CFP/YFP FRET pair would be greatly appreciated.
>
> Kind regards
> Jen
> --
> Jennifer Clarke BSc (Hons) PhD
> Research Associate, Anatomy and Histology
> Centre for Neuroscience, School of Medicine
> &
> Facility Manager, Optical Microscopy Suite, Flinders Microscopy
>
> Flinders University
> GPO Box 2100, Adelaide 5001
> Phone: 61 8 8204 6454/ 61 8 8204 6637
> Email: [log in to unmask]
>
>
> ________________________________________
> From: Confocal Microscopy List [[log in to unmask]] On
> Behalf Of Craig Brideau [[log in to unmask]]
> Sent: Friday, 11 November 2011 5:03 AM
> To: [log in to unmask]
> Subject: Re: FRET in the time of DPSS
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> There are also the new direct diode lasers at 520nm if you can handle
a 6nm
> red wavelength shift from 514. They've only been around for about a
year
> but many manufacturers have lumped them in with their direct-diode
product
> lines and they look pretty decent.
>
> Craig
>
>
>
> On Thu, Nov 10, 2011 at 6:08 AM, Tim Feinstein <[log in to unmask]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi guys (and Guy),
> >
> > Many thanks for the feedback. I like the 505 laser idea in
particular.
> > As long as it excites 488 fluorophores well enough it could be
exactly
> > what I need. Does anybody have experience with using that in place
of a
> > 488 line?
> >
> > All the best,
> >
> >
> > Tim
> >
> > Sent from my iPad
> >
> > On Nov 10, 2011, at 1:57 AM, Guy Cox <[log in to unmask]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > The Sapphire series lasers are OPS (Optically Pumped
Semiconductor) not
> > > DPSS but, you are right, they do indeed have a 514nm version. I
don't
> > > know why I didn't find it, but did find the 505, when I searched
this
> > > morning. I still think that the 505 might be exactly what Timothy
> > > needs, though, since he doesn't want to buy an extra laser.
> > >
> > > I have also been contacted off-list and told that Spectra-Physics
have
> a
> > > 515nm DPSS which is close enough to make no difference. I've no
idea
> > > what the lasing crystal is.
> > >
> > > Guy
> > >
> > > Optical Imaging Techniques in Cell Biology
> > > by Guy Cox CRC Press / Taylor & Francis
> > > http://www.guycox.com/optical.htm
> > > ______________________________________________
> > > Associate Professor Guy Cox, MA, DPhil(Oxon)
> > > Australian Centre for Microscopy & Microanalysis,
> > > Madsen Building F09, University of Sydney, NSW 2006
> > >
> > > Phone +61 2 9351 3176 Fax +61 2 9351 7682
> > > Mobile 0413 281 861
> > > ______________________________________________
> > > http://www.guycox.net
> > >
> > >
> > >
> > > -----Original Message-----
> > > From: Confocal Microscopy List [mailto:
> [log in to unmask]]
> > > On Behalf Of samuel connell
> > > Sent: Thursday, 10 November 2011 1:57 PM
> > > To: [log in to unmask]
> > > Subject: Re: FRET in the time of DPSS
> > >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Industry Response:
> > >
> > > There absolutely is a 514 DPSS available from 20 mW up to 150 mW
> > > available
> > > in the Sapphire form factor from Coherent. We use this laser,
often in
> > > conjunction with the 445 CUBE in our LaserStack for CFP/YFP (and
their
> > > newer brothers and sisters in FP evolution) imaging for FRET or
> > > otherwise.
> > >
> > > ------------------------
> > > Samuel A. Connell
> > > Sales Manager
> > > Pacific Region-North America
> > > Intelligent Imaging Innovations, Inc
> > > 3250 Ocean Park Blvd, Suite 202
> > > Santa Monica, CA 90405
> > > Cell: (858) 692-4510
> > > [log in to unmask]
> > >
> > >
> > > On Wed, Nov 9, 2011 at 4:21 PM, Damir Sudar <[log in to unmask]>
wrote:
> > >
> > >> *****
> > >> To join, leave or search the confocal microscopy listserv, go to:
> > >>
> > > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
> http://lists.umn
> > > .edu/cgi-bin/wa?A0=confocalmicroscopy>
> > >> *****
> > >>
> > >> See: http://www.cobolt.se/**coboltfandango515nm.html?**
> > >>
> > > gclid=CPCOueXlqqwCFQyEhwodQn-**aAw<
> http://www.cobolt.se/coboltfandango51
> > > 5nm.html?gclid=CPCOueXlqqwCFQyEhwodQn-aAw>
> > >>
> > >> No connection, just considering about switching away from our old
Ar
> > > Ion
> > >> as well.
> > >>
> > >> - Damir
> > >>
> > >>
> > >> On 11/9/2011 3:36 PM, Guy Cox wrote:
> > >>
> > >>> *****
> > >>> To join, leave or search the confocal microscopy listserv, go
to:
> > >>>
> > > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
> http://lists.umn
> > > .edu/cgi-bin/wa?A0=confocalmicroscopy>
> > >>> *****
> > >>>
> > >>> Are you sure there is a 514nm DPSS? I think that wavelength
would
> > > have
> > >>> to be an OPS and I don't know anyone who makes one. Coherent do
make
> > > a
> > >>> 505nm OPS which is intended to stand in for both the 488& 514
lines
> > > of
> > >>>
> > >>> an Argon laser, and I rather suspect that this could be exactly
what
> > > you
> > >>> need. But I have no experience of it. (I do have a Coherent
488nm
> > >>> OPS).
> > >>>
> > >>> Guy
> > >>>
> > >>> Optical Imaging Techniques in Cell Biology
> > >>> by Guy Cox CRC Press / Taylor& Francis
> > >>>
> > >>>
> > >
http://www.guycox.com/optical.**htm<http://www.guycox.com/optical.htm>
> > >>> ______________________________**________________
> > >>> Associate Professor Guy Cox, MA, DPhil(Oxon)
> > >>> Australian Centre for Microscopy& Microanalysis,
> > >>>
> > >>> Madsen Building F09, University of Sydney, NSW 2006
> > >>>
> > >>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> > >>> Mobile 0413 281 861
> > >>> ______________________________**________________
> > >>> http://www.guycox.net
> > >>>
> > >>>
> > >>>
> > >>> -----Original Message-----
> > >>> From: Confocal Microscopy List
> > > [mailto:CONFOCALMICROSCOPY@**LISTS.UMN.EDU
> <[log in to unmask]
> > > EDU>
> > >>> ]
> > >>> On Behalf Of Tim Feinstein
> > >>> Sent: Thursday, 10 November 2011 7:34 AM
> > >>> To: [log in to unmask]**EDU
> > > <[log in to unmask]>
> > >>> Subject: FRET in the time of DPSS
> > >>>
> > >>> *****
> > >>> To join, leave or search the confocal microscopy listserv, go
to:
> > >>>
> > > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
> http://lists.umn
> > > .edu/cgi-bin/wa?A0=confocalmicroscopy>
> > >>> *****
> > >>>
> > >>> Hello all,
> > >>>
> > >>> We want to spec a four-laser launch for a new live cell system
that
> > > will
> > >>> handle both CFP/YFP FRET and red/green imaging. However, I am
sad
> > > to
> > >>> see that gas lasers are no longer speccable and so the freebie
514
> > > laser
> > >>> line is gone. We would therefore have to spec a 514 DPSS and
forego
> > > the
> > >>> far-red line.
> > >>>
> > >>> I was wondering whether there is a way to do more (or at least
the
> > > same)
> > >>> with less. 488 nm excites YFP well enough, so in theory I could
> > > image
> > >>> CFP/YFP using a scan head dichroic with cutouts for 442 and 488
nm
> > > laser
> > >>> lines. In my experience 442 nm laser excitation (via TIRF)
causes
> > >>> negligible YFP excitation and 488 nm does not excite CFP, so it
is
> > >>> possible that I could gain speed by passing everything through a
> > > single
> > >>> broad bandpass filter (e.g., 455-550 nm) and alternate
excitations.
> > >>> Assuming that cross-talk is not a problem, the most significant
cost
> > >>> would be that I lose a decent chunk of CFP emission to the scan
head
> > >>> dichroic, but in return I gain a 641 nm laser.
> > >>>
> > >>> Has anyone tried this? Any feedback on or off-list would be
much
> > >>> appreciated.
> > >>>
> > >>> Thanks and all the best,
> > >>>
> > >>>
> > >>> TF
> > >>>
> > >>> Timothy Feinstein, PhD
> > >>> Postdoctoral Fellow
> > >>> Laboratory for GPCR Biology
> > >>> Dept. of Pharmacology& Chemical Biology
> > >>>
> > >>> University of Pittsburgh, School of Medicine
> > >>> BST W1301, 200 Lothrop St.
> > >>> Pittsburgh, PA 15261
> > >>>
> > >>> -----
> > >>> No virus found in this message.
> > >>> Checked by AVG - www.avg.com
> > >>> Version: 10.0.1411 / Virus Database: 2092/4005 - Release Date:
> > > 11/08/11
> > >>>
> > >>
> > >> --
> > >> Damir Sudar - Staff Scientist and Deputy for Technology
> > >> Lawrence Berkeley Laboratory / Life Sciences Division
> > >> One Cyclotron Road, MS 977, Berkeley, CA 94720, USA
> > >> T: 510/486-5346 - F: 510/486-5586 - E: [log in to unmask]
> > >> WWW: http://www.lbl.gov/lsd/People_**&_Organization/Scientific_**
> > >>
> > > Staff_Directory/Sudar_Lab.html<
> http://www.lbl.gov/lsd/People_&_Organizat
> > > ion/Scientific_Staff_Directory/Sudar_Lab.html>
> > >>
> > >
> > > -----
> > > No virus found in this message.
> > > Checked by AVG - www.avg.com
> > > Version: 10.0.1411 / Virus Database: 2092/4006 - Release Date:
11/09/11
> >
>
>
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