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Hello all:

I am collecting in situ psfs from Arabdiopsis leaves.For me it is
impossible to collect PSF's in z direction  as I will also capture the
leaf structure too and I only collect data in XY plane.
I am wondering if it is possible to do 2D deconvolution by these
collected PSF s with Huygens software(we haven't get the soft ware yet
we would like to try this with the test licence of the software).

I really appreciate any information and any idea.

Sarah