Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Double check the manual to see that this really gives you more dynamic 
range and not just finer resolution of the same signal range.

Dale

Jacqui Ross wrote:
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi Maria,
>  
> What happens if you switch to 12bit imaging instead of 8bit? This would 
> give you a greater dynamic range so may help with the saturation problem.
>  
> Kind regards,
>  
> Jacqui
>  
> 
> *Jacqueline Ross *
> 
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit 
> School of Medical Sciences 
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland, NEW ZEALAND
> 
> Tel: 64 9 373 7599 Ext 87438
> Fax: 64 9 373 7484
> 
> http://www.health.auckland.ac.nz/biru/ 
> <blocked::http://www.health.auckland.ac.nz/biru/>
> 
>  
> 
> ------------------------------------------------------------------------
> *From:* Confocal Microscopy List [mailto:[log in to unmask]] 
> *On Behalf Of *Maria Jimena Ortega
> *Sent:* Wednesday, 12 March 2008 7:34 a.m.
> *To:* [log in to unmask]
> *Subject:* 510 meta: scan speed, saturation & stripes
> 
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hello,
> 
>  
> 
> I am having a situation with a new 510 Meta from Zeiss and would like to 
> receive some feedback from other users…
> 
>  
> 
> First thing I notice is that the intensity level varies a lot with scan 
> speed (from 3.2 usec/pixel to .64 usec/pixel).  So, parameters choosed 
> at a speed doesn`t work in the other…  it really  annoying!
> 
>  
> 
> The other situation is that when I am working at high speeds (0.8 – 1.64 
> usec/pix), average 4 line, if my image has saturating conditions ( I 
> =255, 8 bit) I see some regular “stripes”.  
> 
> Zeiss tells there is a security system (to protect the PMT), but I do 
> not understand why I see it in some speeds and not others, or why I 
> don`t get them in my Pascal…  any clue?
> 
> The situation is that I need to saturate some parts of my sample in 
> order to see the parts I am interested in!!  What should I do?
> 
>  
> 
> The also tell me not to make average and slow down speed… but I don`t 
> get the same results… Can someone help me in this scan speed vs average 
> discussion?
> 
>  
> 
>  
> 
> Any clue would be gratefully received!
> 
>  
> 
> Maria
>