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Hello to all,

I am preparing for our first internal workshop on LSCM and I would appreciate a
few clarifications (and some help).

1- I am looking for the publication or book (or any reference) which shows that
a correctly acquired image has a histogram distribution of pixel intensity with
a count of 0 at intensities 0 and 255.  I know I read this somewhere...

2- I have a technical question about ensuring that the detector is not saturated
during acquisition.  I usually set the laser power and detector gain with a 1
second scan (512x512 pixels) and then select averaging to collect the final
image.  If I were to select a longer dwell time during set up, I may select a
different gain setting for the detector because saturated pixels are less
probable (but did I saturate the detector during the scan...). So how is the
signal being processed for each pixel?

3- I have a further technical question about the Zeiss LSM410.  Are line and
frame averaging both using the Kalman averaging approach?

Regards,
Sophie
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Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
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Saskatchewan Structural Sciences Centre
University of Saskatchewan
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