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Hi,
Following up on the effect of pulse-width and photo-toxicity, I would also have a look at the
following paper from the Beaurepaire lab:
Débarre D, Olivier N, Supatto W, Beaurepaire E (2014) Mitigating Phototoxicity during
Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range.
PLoS ONE 9(8): e104250. doi:10.1371/journal.pone.0104250
Principles should be applicable to other organisms (me thinks)
Debbi