CONFOCALMICROSCOPY Archives

November 2014

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Subject:
From:
Craig Brideau <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 12 Nov 2014 16:56:23 -0700
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Really good early work. I basically redesigned the prisms but used the same
compressor design as Soeller. The key change I made for my own work was to
use a higher-index glass so the prisms didn't have to be so far apart to
achieve a given -GVD. The trick was to balance out the Abbe value for the
bandwidth of the laser so I induced mainly second order but not much third
order dispersion.

Craig


On Wed, Nov 12, 2014 at 4:06 PM, Mark Cannell <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi
>
> Have a read of this, I think it gives a relatively easy to understand
> description of the problem and its solution:
> Soeller, C. & Cannell, M.B., 1996. Construction of a two-photon microscope
> and optimisation of illumination pulse duration. Pflügers Archiv : European
> journal of physiology, 432(3), pp.555–561.
>
> HTH
>
> Cheers
>
>
>
> On 12/11/2014, at 8:44 pm, Michael Moore <[log in to unmask]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > I'm a new member to the confocal listserv
> >
> > Can someone explain GDD in regards to multiphoton imaging?
> > And how is GDD adjusted.
> >
> > We currently have a Coherent chameleon vision II that has GDD.
> >
> > I have been told conflicting instructions on how the GDD is adjusted or
> > used for imaging.
> >
> > Maybe I just don't understand how GDD works.
> >
> > for one, is GDD adjusted for each objective used?  is it independent of
> the
> > objective?
> >
> > Thanks to all who reply.
> >
> > Mike
>
> Mark  B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology &  Pharmacology
> Medical Sciences Building
> University of Bristol
> Bristol
> BS8 1TD UK
>
> [log in to unmask]
>

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