Does anyone have ideas or pointers to references on fluorescent temperature
probes, for measuring intracellular  changes in temperature?
 
Once upong a time I found that sulforhodamine-B (SRB) showed an easily measured
temperature dependence in solution around 25-35 degrees C. This dependence did
not exist with sulforhodamine 101 (SR101). I assumed that this is because in
SR101 the nitrogens are rigidized by two aliphatic rings each, whereas in SRB
the nitrogens can rotate.
 
I thought to use this phenomenon to look at temperature changes, but I realize
that I will probably bleach the dye and this will hide the affect of
temperature change. So I would be better off with a dye that changes its
spectral characteristics as a function of temperature, and allows for a
wavelength ratio measurement. Sensitivity of 0.5 degree C will do for starters.
 
Any ideas will be appreciated.
 
--aryeh
 
--
Aryeh Weiss                          | email:     [log in to unmask]
Department of Electronics            |
Jerusalem College of Technology      | phone:     972-2-751146
POB 16031                            | FAX:       972-2-422075
Jerusalem, Israel                    | ham radio: 4X1PB/KA1PB