CONFOCALMICROSCOPY Archives

March 2001

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Subject:
GFP in Drosophila
From:
"Larry D. Ackerman" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 23 Mar 2001 10:54:00 -0800
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In all the drosophila GFP experiments did you devitellinize with methanol?
Did you ever try any experiments devitellinizing by hand in 1 or 2%
formaldehyde? (I hope not to have to do that.) We can image GFP in living
embryos but of course sometimes we would like a double or triple label
using antibodies and the anti-GFP antibodies we have tried do not work well.


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751  FAX (415) 476-5774
[log in to unmask]
http://www.ucsf.edu/janlab

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