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| Date: | Wed, 4 Nov 2009 08:07:19 -0600 |
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Alexa405 has been very dim and bleach-prone in my hands. I think if
you call Invitrogen they might tell you that "Marina blue" is a better
option, I remember Mike Ignatius posting something to that effect in
the past couple of years.
Good luck.
-Esteban
On Tue, Nov 3, 2009 at 4:16 PM, Andrea Hooper <[log in to unmask]> wrote:
> Dear All,
> I need some advice in setting up for a very important experiment coming up.
> Has anyone used the fluorophore PerCP CY5.5 with success for confocal
> microscopy? Any experience with autofluorescence in this wavelength or
> whether this fluorophore is a severe bleacher etc.?
> I want to do 5 or 6 color on a Zeiss LSM710 and am thinking about the
> following, I would love your feedback.
>
> Alexa405
> Alexa430
> GFP
> RFP
> Alexa633
> PerCP CY5.5
>
> Thank you in advance,
> Andrea
> --------------------------------
> Andrea T. Hooper, PhD
> [log in to unmask]
--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO 65211
http://www.biotech.missouri.edu/mcc
(573)882-4895
(573)884-9676 fax
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