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Hi Shanna,

Yes, as Michael mentioned already, you can do a correction with ZEN 2009.

It's called Auto Z Brightness correction and you can find it in the Z stack dialogue window at the bottom. It's linear and is very easy to set up. Instructions are given on page 4-71 of the full manual that I have. I have a copy in PDF so if you don't have this, let me know and I will send it to your email address.

Kind regards,

Jacqui


Jacqueline Ross 
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences 
The University of Auckland 
Private Bag 92019 
Auckland 1142, NEW ZEALAND 

Tel: 64 9 923 7438 
Fax: 64 9 373 7484 

http://www.fmhs.auckland.ac.nz/sms/biru/

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Shanna Banman
Sent: Wednesday, 5 September 2012 10:57 a.m.
To: [log in to unmask]
Subject: Compensation for fluorescence loss Zeiss LSM 710 2-photon

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Hello all,

Does anyone know if there is a setting in Zen2009 for Zeiss LSM 710 that compensates for loss of fluorescence signal detection as you penetrate deeper into the sample?  i.e. as you image deeper (100 um), the system automatically increases the laser intensity?
Thank you!!

Shanna Banman