CONFOCALMICROSCOPY Archives

March 2008

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Subject:
Re: DNA dyes
From:
Charles Matthew Blaha <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 12 Mar 2008 13:26:39 -0700
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hey Carl,

I stained human mesenchymal stem cells with Hoech 33342.  I then  
imaged them with time lapse microscopy.  The cells did not die and the  
nuclei stained really well.  However, the cells did not divide.

charlie

Quoting Carl Boswell <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear all,
> What is the consensus on using either DAPI or Hoechst staining on live
> cells?  Are there effects on mitosis and/or chromosome movement?
> Thanks,
>
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709

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