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March 2008

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Subject:
Re: Fluorophore bleaching by excitation light sources
From:
Stanislav Vitha <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 12 Mar 2008 16:27:57 -0400
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Regarding the difference between LED illumination and Hg lamp - 
Perhaps if the illumination is not continuous but is set up for strobe 
operation of the LED, this could allow dark state relaxation an prolong 
the life of the fluorophore (I am not sure the same would apply to the 
life of the cell). - The time between light pulses should be more than one 
microscecond.
reference:   
Gerald Donnert, Christian Eggeling & Stefan W Hell: Major signal increase 
in fluorescence microscopy through dark-state relaxation. Nature Methods - 
4, 81 - 86 (2007)


Stan Vitha

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