>I wanted to know what people do to create RGB movies from their Leica_NT
>3D or timelapse files.  These files are concatenated TIFF (I guess
>similar to NIH Image stacks) that store the 3 channels in G1...Gn,
>R1...Rn, B1...Bn order.
>
>We want to create colour QT movies, each frame combining the 3 equvalent
>RGB frames (ie G1R1B1...GnRnBn).
>
>I've tried different options to get there.  So far the quickest is using
>Imaris to export the data as series of RGB TIFF, and then use Graphic
>Converter (on a Mac) to create a colour QT movie from these files.
>
>I want to bypass the IMARIS step. Is there a program that can be told
>that the serie of tiff images presented to it are actually 2 or 3
>channels and that they should be combined together?
>
>If tried presenting the original Leica_NT data to graphic converter.
>It converts the TIFF series to QT, but it leaves the channels one after the
>other, in sequence.
>
>Ideally, I want to tell the program that out of the 30 images in that
>file, 1-10 are Green, 11-20 are Red and 21-30 are Blue, so thay can be
>combined accordingly.
>
>Are any plug-ins for Photoshop or Graphic Converter that could do what I
>want?
>
>Thanks,
>
>****************************************************************************
>
>Dr. Stamatis Pagakis                               [log in to unmask]
>Confocal and Image Analysis Laboratory              Tel: +44 (0)181 913 8675
>Division of Membrane Biology                Switchboard: 0181 9593666 x2621
>National Institute for Medical Research         Message: x2219, x2622
>The Ridgeway, Mill Hill, London NW7 1AA             FAX: +44 (0)181 906 4477

At some cost, you can use IP Lab Spectrum from Signal Analytics
(www.iplab.com/sac/), as I'm doing. With IP Lab,in order to perform this
and similar jobs, you can create a script with names and details of your
input images and the name plus characteristics of the desired QT output
movie.

For free, you can probably convert TIFFs to PICTs (with Graphic Converter
and some effort) and use some of the existing utilities for PICTs to Movie
conversion. For this, see one or more of the many Mac sw sites, on the net.




Pier Alberto Benedetti
Istituto di Biofisica CNR
Via S. Lorenzo 26
56127 Pisa (Italy)

Tel: +39-50-513241
Fax: +39-50-553501
e-mail: [log in to unmask]