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June 2007

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Confocal Microscopy List <[log in to unmask]>
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Fri, 1 Jun 2007 09:12:15 -0500
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Confocal Microscopy List <[log in to unmask]>
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Re: DIC Issue
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Martin Wessendorf <[log in to unmask]>
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Claire Brown wrote:

> Just to clarify we see this difference in focal plane when we are 
> looking in the scope by eye and not via the confocal scanner. We have 
> also verified that we don’t see it with our other objectives so I’m 
> thinking now that there may be an issue with our 63x objective.

Check for a dirty objective.  I think I've seen junk on the glass 
introduce chromatic aberration.

Good luck!

Martin
-- 
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu

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