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Date: | Wed, 12 Feb 1997 08:56:37 +0000 |
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> I have been told that confocal-like images with optical sectioning
>capability can be obtained without using laser confocal microscope hardware.
>One obtains images using a standard microscope and ccd camera as one steps
>along in z (ie numerous changes in focus). These blurred images are
>processed together along with the point spread function for the optical
>system to produce in-focus images at the numerous z-planes. Can someone
>tell me:
>
>1. Where I can find a good description of this approach.
>2. Where I can obtain (free? buy?) the deconvolution algorithm.
>3. If there is an accepted name for this approach.
>4. What are the tradeoffs between the hardware and software approaches
> in x, y and z resolution and acquisition time.
>
>Thank you in advance for any asssistance
>
> Lauren M. Peterson (313) 994-1200 Ext 2284
> ERIM (313) 994-5704 FAX
> Letter Address: P.O.Box 134001
> Ann Arbor, MI 48113-4001
> Package Address: 1975 Green Rd.
> Ann Arbor, MI 48105-2554
> Electronic Mail -- Internet:
> [log in to unmask]
> [log in to unmask]
You might want to look at:
Hai-Shan Wu et al.
A focusing algorithm for high magnification cell imaging.
J. Micr., 184(2), 133-42, Nov 1996.
Not quite what you are looking for perhaps but related. It describes a
method for taking a series of images at different focus settings (and thus
different depths), and then recombining into a single, in-focus image.
Regards,
---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: [log in to unmask]
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
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