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June 2000

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Subject:
Re: electroporation of indicators
From:
Milton Charlton <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 23 Jun 2000 14:32:49 -0400
Content-Type:
TEXT/PLAIN
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TEXT/PLAIN (8 lines) 
Several studies showed AM loading of Ca indicators and chelators at room
temperature for instance at
frog neuromuscular junction.  In some cells co-application of an anion
pump blocker like probenecid will help retain tetra-anionic dyes and
chelators after the AMs are removed.
Milt Charlton
University of Toronto

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