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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
On 19/02/2008, at 3:41 AM, Michael Weber wrote:
> Hi David,
>
> the system measures the amount of fluorescence signal per pixel and
> controls the AOTF for this pixel based on the measured value. So if
> there is no light coming to the detector, laser will be restricted
> from illuminating the sample. Or, if the detector gets close to
> saturation, laser will be restricted as well. This should reduce
> photobleaching and phototoxicity also in out-of-focus areas. That's
> at least how I understand this.
Does it work with the AOTF on the A1?
When I enquired about it on the C1 I was told it was only available
with the AOM on the 3-laser board, not the AOTF on the 4 laser board?
Regards,
Adrian Smith
Centenary Institute, Sydney, Australia
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