Hi Cameron,

Thanks for the infos. From what I tested on friday and from what you 
wrote, it's seams that for live cell imaging, Lysotracker won't be a good 
tool at all.

Thanks,

JP



> Hi JP,

> I wouldn't be concerned about 3% power, in fact I would think that 
> is quite low. I have some users using lyotracker red (and green) in 
> whole zebrafish and they routinely are setting the 559 laser at 
> around 10-20%. This is on an FV1000 system with the 20x NA0.95 
> dipping objective.

> I have a feeling the lysotracker dyes are a bit weak, i would be 
> interested in hearing other peoples experience as well. We have 
> tried the blue/yellow in the past and have given up on it because 
> while the signal is there, it is very weak and required high laser 
> power and long scan times to get a decent image.

> Cheers

> 
> Cam

> 
> Cameron J. Nowell
> Microscpy Manager
> Central Resource for Advanced Microscopy
> Ludwig Insttue for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA

> Office: +61 3 9341 3155
> Mobile: +61422882700
> Fax: +61 3 9341 3104

> http://www.ludwig.edu.au/branch/research/platform/microscopy.htm

> 
> ________________________________

> From: Confocal Microscopy List on behalf of Jean-Pierre CLAMME
> Sent: Sat 30/05/2009 10:58 AM
> To: [log in to unmask]
> Subject: Lysotracker Red

> 
> Hello,

> I was wondering if some of you had experience with the LysoTracker 
> Red DND-99 and maybe even on the Fluoview 1000.
> I tried it out today and I was surprised by the weakness if the 
> signal.  I had to use over 3% of my 559 Laser line (I generally use 
> 0.3 to 0.5% for Cy3 labelled particles detected on the same channel).
> It would be helpful if I could get some feed backs from your 
> experience with this dye.

> Thank you in advance,

> JP

> 
> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
> Jean-Pierre CLAMME, PhD
> Senior Scientist
> Nitto Denko Technical
> 501 Via Del Monte
> Oceanside, CA 92058
> E-mail: [log in to unmask]
> Phone: +760.435.7065