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Dear all,

I would like to use a ziess axiovert  microscope (LSM 510 confocal unit)
coupled to a spectra physics tsunami multiphoton laser to image the shg
signal from collagen fibres in heart valves.  

I am wondering what is the best way to do this and can anyone give
comments / recommendations on how our  existing machine may be
retrofitted for this task.

Thanks

Peter Owens 


__________________________________
Dr. Peter Owens
Centre for Microscopy and Imaging and NBIPI
National University of Ireland Galway
Landline: 0035391494036
Mobile:    00353863326749
------------------------------------------------------

 

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Mark Cannell
Sent: 02 May 2012 17:21
To: [log in to unmask]
Subject: Re: confocal PMT intermitently dies

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Check connectors...  Unplug and replug all connectors. If that doesn't
work then its most likely electronic in the pmt3 power supply or pmt3
preamp stages...

Hope this helps
Mark

On 2/05/2012, at 4:22 PM, Arvydas Matiukas wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear list,
> 
> 
> Ch3 PMT intermitently dies on our LSM510 system. After 2-4 hrs of 
> powering on, all image pixels turn to  zero intensity no matter what 
> are laser power, pinhole, filter, and gain settings are used.
> Cold reboot does not fix the problem though no error messages are 
> reported. The single fix so far is to let the system cool down for 
> several hours then it is again working for few more hours.
> Surprisingly all cooling fans are spinning normally as well as  room 
> conditioning system.
> 
> 
> 
> 
> We are off service contract so any feedback/advice what could be wrong

> and how to fix it is greatly appreciated.
> 
> 
> Thank you,
> Arvydas
> 
> 
> 
> 
> 
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Imaging Core Facility Department of 
> Pharmacology SUNY Upstate Medical University
> 766 Irving Ave., WH 3159
> Syracuse, NY 13210
> tel.: 315-464-7997
> fax: 315-464-8014
> email: [log in to unmask]