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Dear Martin,

 Re: non-CFP-like FRET pairs: in our (and many other people's) hands ,
GFP-mCherry works very well: It's based on the most optimised FPs of all
(GFP) - monomeric, single-exponential decay, bright, photostable, ..... - ,
efficiency is good yet the spectra nicely separated for stimulated emission.

Best,

Martin
###############################
Martin Spitaler, PhD
FILM - Facility for Imaging by Light Microscopy
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK

Tel. +44-(0)20-759-42023
E-mail [log in to unmask]
Website: http://imperial.ac.uk/imagingfacility 

On Mon, 6 Aug 2012 02:31:43 -0500, Martin Offterdinger
<[log in to unmask]> wrote:
>
>Dear all
>Thanks a lot for your input, which is very much appreciated, indeed.
>What surprises me a bit, though, is the focus on the CFP-YFP system.
>One big problem with that pair has not yet been discussed at all: The
generation 
>of a CFP-like photoproduct in acceptor bleaching experiments. I have seen this 
>effect myself and it was very strong! So are there any further comments on
this 
>or improved YFP version that don't photoconvert to CFP-like emitting
species...?
>
>Thanks again