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June 2011

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Subject:
From:
Christian Götze <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 29 Jun 2011 21:35:02 +0200
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*****
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*** Commercial response ***

Hi Kris,

what does "about 2 gigabytes" mean? Is it below 2 GB or above? Although 
the TIFF standard uses 32 Bit pointers and thus defines TIFFs up to 4 
GB, several of the TIFF libraries available use "signed pointers" and 
therefore can't open stacks larger than 2 GB.

But of course the file could be simply corrupted and therefore can't be 
read by Metamorph.

Perhaps you should try to open it with other software (ImageJ, Photoshop).

In our applications we use a special modded TIFF library to open large 
TIFFs. You can download a trial at

http://www.arivis.com/en/arivis-Vision-4D

If your problems persist we could have a look on your data. Just write a 
mail if interested.

Best,
Christian

Am 29.06.2011 21:06, schrieb krisford:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
>
> I took a live streaming in Metamorph and saved it in a TIFF video file.  I
> tried to open it later in Metamorph and got the message unable to open.
> The file was about 2 gigabytes and 5400 frames.  I'm using 64-Bit Windows
> and have about 3 gigs of RAM.  Can anyone help?  It would be sincerely
> appreciated.
>
> Thanks,
> Kris

-- 
Christian Götze

arivis - Multiple Image Tools GmbH
Kröpeliner Straße 54, D-18055 Rostock

Tel : +49 381 461393 11
Fax : +49 381 46139399
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