Hi Richard,
        We are considering microinjection for our studies and would
     therefore be grateful for a copy of replies you receive.  Thank you in
     advance.  Steve Gaffin
     US Army Research Institute for Environmental Medicine
     Natick MA
     [log in to unmask]


______________________________ Reply Separator _________________________________
Subject: microinjection
Author:  Confocal Microscopy List <[log in to unmask]> at
Internet-Mail
Date:    6/5/97 1:10 PM


hello,

Does anybody have experience in microinjecting substances/ cDNAs into
cells and not only detect the effects in fixed specimen but also to
observe the effects in living cell culture at 37 C?
We co-inject Texas red-dextran / GFP and observe effects already in
control injected cells. How do we minimise the mechanical stress the cell
have to undergo? Buffer suggestions, injection conditions etc. are
extremely welcome.


Richard Wubbolts/Mar Fernadez-Borja
Netherlands Cancer Institute
Plesmanlaan 121
1066 CX Amsterdam
tel:  *31-20-5121973
fax: *31-20 5121989
e_mail: [log in to unmask]