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January 2008

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Confocal Microscopy List <[log in to unmask]>
Subject:
Green fluorescing counterstain?
From:
Stanislav Vitha <[log in to unmask]>
Date:
Wed, 30 Jan 2008 19:02:22 -0500
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Search the CONFOCAL archive at
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Dear confocalists,
do you have suggestions for a general counterstain that would fluoresce in 
green? 
I am doing 3D imaging of large number of pollen samples, and would like to 
increase the signal levels so that my acquisition times become more 
reasonable.

The pollen has been processed (cytoplasm removed), so I am trying to stain 
the shell (exine/intine) and am not interested in the cytoplasm.

Rhodamine B staining worked fine, but the emission is in red. I am after 
shorter wavelength signal for best resolution.

Thank you!


Stan Vitha

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