I got the Syto dye set a while ago and none of them stained Dictyostelium
nuclei.  I got punctate cytoplasmic staining which I have never
investigated further.  Dave
 
>Has anyone on the list had experience with Syto 17 from Molecular Probes ?
>
>I do not seem to get nuclear staining with mammalian cells.  I incubated 200
>micromolar dye at 37 deg C for 30-45 minutes in media and buffer without
>Phosphate and the dye appears all in the cytoplasm.
>
>Am I missing something in technique ?
>
>
>Mike Esterman
>Scientific Imaging Center
>Lilly Research Labs
>Indianapolis IN 46285
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Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
U-125
Storrs, CT 06269
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