True

thanks all

Valeria

Carl Boswell wrote:
> Wow.  Ask and ye shall receive.
> Thanks Steve.
>
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709
> ----- Original Message ----- From: "Stephen Bunnell" 
> <[log in to unmask]>
> To: <[log in to unmask]>
> Sent: Tuesday, April 07, 2009 4:53 PM
> Subject: Re: GFP and mCherry: Hands on Data
>
>
> As noted, DsRed, mRFP1, mCherry, mStrawberry, and tdTomato are derived 
> from
> a common source.
>
> Of these, the rabbit polyclonal anti-DsRed antibody from BD, #632397
> recognizes all but tdTomato by western blotting. It will also IP these
> proteins.
>
> Similarly, GFP, EGFP, CFP, Cerulean, YFP, Citrine, and Venus are derived
> from a common source, as are the common monomeric variants of the same 
> (the
> A206K mutation is most typical).
>
> We have confirmed that the JL-8 monoclonal from BD recognizes Venus,
> Cerulean, CFP, YFP, mCFP, mYFP, and EGFP by western blotting. We have not
> seen it crossreact against DsRed, mRP1, or mCherry. For IPs we use the 
> Abcam
> antibody 'ab290', which works well for EGFP, YFP, mYFP, CFP, and mCFP, 
> and
> does not capture DsRed, mRFP1, or mCherry.
>
> Good luck,
>
>    -Steve
>
>
> On 4/7/09 8:19 AM, "Valeria Berno" <[log in to unmask]> wrote:
>
>> Quick question:
>>
>> Does someone knows if the antibody anti-GFP would cross-react also with
>> the mCherry?
>>
>> I assume the answer is no but i cannot find any specification. I could
>> only found no cross-reactivity of DsRed antibodies with GFP......
>>
>> Thanks in advance
>>
>> Valeria
>
> **************************************************************************** 
>
> Stephen C. Bunnell, Ph.D.
> Assistant Professor
> Tufts University Medical School
> Department of Pathology
> Jaharis Bldg., Room 512
> 150 Harrison Ave.
> Boston, MA 02111
>
> Phone: (617) 636-2174
> Fax:   (617) 636-2990
> Email: [log in to unmask]
>