Dear Confocalists !
The Trypan bleu/crystal violet quenching method was devoloped for
microscopy  more than 15 y ago by Dr. J. Hed, then at our laboratory. He
and his collaborators have later applied it for flow cytometry as well.
karl-Eric Magnusson

Philip Oshel wrote:

> Dr. Briggs,
>
> This is a nice method, and would make a good tech tip or short
> article for Microscopy Today. Would you be interested in writing it?
> It also reads like you get some excellent micrographs from your
> specimens. We are always looking for good color images for our cover
> (horizontal format), and would like to ask if you would be interested
> in sending in an image.
>
> Thank you for your attention.
>
> Phil
>
> >We use heat killed yeast, stained with eosin Y and well rinsed, as
> >our particle for uptake.  This is quenched (after rinsing away the
> >excess/unadherent yeast) by adding 0.2% trypan blue (in saline) to
> >the cell/yeast prep and then viewing.  It is spectacular - any
> >particle that lights up is inside a cell.  I don't know the range of
> >other dyes quenchable by the trypan blue.  I can send more details if
> >you wish.
> >
> >Dick Briggs
>
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