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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Elaine, I've used both DAPI and Phalloidin-rhodamine to stain Schistosoma
mansoni eggs (see Bahia et al. Parasitology, 133: 321-329 2006.
Good luck
Renato
Quoting carl <[log in to unmask]>:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Elaine,
> The Hoechst stains, especially 33342, are quite permeable across live cell
> membrane and into thick-skinned nematodes. Give it a try; from Molecular
> Probes, and probably other sources.
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709
> ----- Original Message -----
> From: Elaine Kunze
> To: [log in to unmask]
> Sent: Tuesday, November 07, 2006 1:08 PM
> Subject: staining worm eggs
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> I am looking for a stain (possibly DNA) that will stain worms so that I can
> count their eggs, in utero, using imaging software. Currently we have tried
> staining with PI, with and without fixation (not bad, not great), YoPro,
> Draq5 (little staining), Vibrant Orange DNA dye (all over the place), PHK26
> for membranes (unimpressive). Since none of these seem to be working really
> well if anyone has any suggestions of what we could use they would be much
> appreciated. I should also add that the worms that we are trying to stain are
> the nematodes Heligmosomoides polygyrus and Syphacia spp., but I am presuming
> that the methods for C. elegans will work for these nematodes too.
>
>
>
>
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