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Elaine, I've used both DAPI and Phalloidin-rhodamine to stain Schistosoma 
mansoni eggs (see Bahia et al. Parasitology, 133: 321-329 2006.

Good luck

Renato

Quoting carl <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hi Elaine,
> The Hoechst stains, especially 33342, are quite permeable across live cell
> membrane and into thick-skinned nematodes.  Give it a try; from Molecular
> Probes, and probably other sources.
> Carl
> 
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709
>   ----- Original Message ----- 
>   From: Elaine Kunze 
>   To: [log in to unmask] 
>   Sent: Tuesday, November 07, 2006 1:08 PM
>   Subject: staining worm eggs
> 
> 
>   Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal 
>   I am looking for a stain (possibly DNA) that will stain worms so that I can
> count their eggs, in utero, using imaging software. Currently we have tried
> staining with PI, with and without fixation (not bad, not great), YoPro,
> Draq5 (little staining), Vibrant Orange DNA dye (all over the place), PHK26
> for membranes (unimpressive). Since none of these seem to be working really
> well if anyone has any suggestions of what we could use they would be much
> appreciated. I should also add that the worms that we are trying to stain are
> the nematodes Heligmosomoides polygyrus and Syphacia spp., but I am presuming
> that the methods for C. elegans will work for these nematodes too.
> 
>    
> 
>