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From: John G. Aghajanian, Ph.D.  <[log in to unmask]>
Fri, 27 Jan 1995 15:11:21 -0400
To: Multiple recipients of list CONFOCAL <[log in to unmask]>
Subject: Anti-fade agents
 
Hello folks,
 
This may sound somewhat trivial but as one just starting out confocaling it
is somewhat pertinent.  Is there a single anti-fade agent that works best
for many/all fluorophores or are there certain anti-fade agents that work
best with particular fluorophores? And, what are they?
 
Thanks in advance,
 
 ___________________________________________________
 |                                                 |
 |   John G. Aghajanian, Ph.D.                     |
 |   Worcester Foundation for Experimental Biology |
 |   222 Maple Avenue                              |
 |   Shrewsbury, MA 01545-2737                     |
 |                                                 |
 |   Tel: 508 842-8921  ext. 147, 161              |
 |   Fax: 598 842-9632                             |
 |   [log in to unmask]                            |
 |                                                 |
 |_________________________________________________|
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We use MOWIOL polyvinyl alcohol as an anti-fading. I think it works o.k.
but it requires preparation (you can store it a -20!C). You can order it or
ask for information to Calbiochem.
 
Susanna
UNITAT DE MICROSCOPIA CONFOCAL
SERVEIS CIENTIFICO-TECNICS.UNIVERSITAT DE BARCELONA
LLUIS SOLE I SABARIS, 1-3 08028 BARCELONA
Tel. 34 3 402 13 52  Fax. 34 3 402 13 98
E-mail: [log in to unmask]