Dear Jon,

why don't you use curry powder? It has nice fluorescence which never bleaches.

Anneliese Schmaus
Klughammer Bio GmbH



2008/11/15 Patrick Van Oostveldt <[log in to unmask]>:
> Dear Jon,
>
> A lot of house hold products show very bride fluorescence. If you take a
> white T-shirt, the whitener in the washing powder will make the contton
> fibers bride fluorescent for UV.
> Most textile dyes are fluorescent also, so you can have choice.
>
> Pollen from plants are also fluorescent. Bye e.g. lilies, give them to your
> love, but take the pollen of before. This will also improve the shelf-live
> of your flowers when put in water. To prepare these pollen, let them dry,
> and mount in immersion oil or non fluorescent glycerine.
>
> Finally the paper of euro's and also very frequently some stamps fluoresce.
> You can check that by illuminating them with blacklight as used in the
> disco.
>
> It is amazing to test all different products, because even the human hair,
> washed with colored shampoo, will show autofluorescence coming from the dye
> in the shampoo, on the other hand, hair from wild animals will in general
> not fluoresce.
>
> Bye,
>
> Patrick
> Dep. Molecular Biotechnology
> Ghent University
> Coupure links 653
> 9000 GENT (Belgium)
>
> Quoting Jonathan M Krupp <[log in to unmask]>:
>
>> Hi:
>>
>> This is a pretty basic and simple question. I need some advice about
>> rounding up some samples to use in an elementary light microscopy class
>> that includes basic fluorescence.
>>
>> I am at a community college and my background is in EM and brightfield
>> LM. I have a passing familiarity with confocal et al but not enough to
>> know much. I maintained a confocal, but never ran experiments or
>> prepared much in the way of samples. Now I have to get something
>> together to demonstrate the fundamentals of fluorescence to students in
>> a new job.
>>
>> I have been relying on chlorophyll autofluorescence up til now, but
>> would like to add anything that would be easy to do. We have a simple
>> scope with filters for FITC, rhodamine, and DAPI, I think. What would
>> be some fool proof, easy to get samples to try?
>>
>> In addition, I would eventually like to add some kind of confocal
>> experience to this class, any ideas about where to find an inexpensive
>> system would be great.
>>
>> Thanks
>>
>> Jon
>> San Joaquin Delta College
>> Stockton, CA 95207
>> [log in to unmask]
>
>
>
> --
> Dep. Moleculaire Biotechnologie
> Coupure links 653
> B 9000 GENT
>
> tel 09 264 5969
> fax 09 264 6219
>