I do not expect any company can provide these details and its involved work. I know Dr. Warren Zipfel, David Wokosin and others measured these parameters. You can visit the web site given below or talk to Warren
http://www.drbio.cornell.edu/personnel/wrz2.html
Hope this helps
Ammasi


Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:[log in to unmask]
http//:www.kcci.virginia.edu
************************
Workshop on FRET Microscopy, March 3-7, 2009
http://www.kcci.virginia.edu/workshop/workshop2009/index.php
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From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Mayandi Sivaguru
Sent: Friday, September 26, 2008 3:11 PM
To: [log in to unmask]
Subject: Re: Pulse broadening in multiphoton micoscopes


Andreas, I do not think it is the responsibility of the laser manufacturer to provide this data, as this data vary with what kind of optics you use. These measurements are typically done by Application Scientists of the microscope vendors using the lasers either from Spectra or Coherent. Perhaps you can directly contact the head of applications of respective scope manufacturer that whether they have ever measured these peak widths directly on the objectives (air, oil and water immersions) using autocorrelators and so on. For example, you can contact Renee Hessling, Head of Applications, Carl Zeiss, Jena for such data from Zeiss ([log in to unmask]).
Shiv


At 12:25 PM 9/26/2008, you wrote:

Short pulses out of an IR laser broaden considerably when travelling through
dispersive medium, e.g. glass lenses and acoustic optic modulator of a
multi-photon microscope. It is often claimed that shorter pulses broaden
more than longer ones. Does anybody know good literature on this? You can
compensate for this by negative prechirp, would the small difference in
initial pulse width between the Spectra Physics and Coherent lasers have an
effect after prechirp and passing through the microscope?

Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: 217.244.2496
[log in to unmask]
http://core.igb.uiuc.edu

<http://core.igb.uiuc.edu/>