Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

For very bright staing, the 543 laser is fine, but I would like to second
the comment below, except that our backup solution is the BioRad Radiance
2000 or cooled CCD camera with deconvolution.  And in defense of Leica,
this problem is not Leica's; it is simply the low power output of the
laser.  The alternative would be the Kr/Ar laser which is fickle.

Forget about using the 543 laser for acceptor bleaching FRET.


> We haven't made that change but we have the HeNe 543 currently on our
> SP2 and are really disappointed with its performance.
>
> The SP2  is a great machine but the poor performance of the HeNe 543
> (due to the low power) means that we often need to swap people back onto
> the old TCS 4D which has an ArKr laser if they have a "red" label. The
> only other option is to open up the pinhole and see whether getting a
> thicker optical slice helps.
>