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Dear Listers,

we are about to fix the details on the equipment for several microscopes 
for a new core facility. One thing that came up was the excitation light 
source for conventional fluorescence for confocals.

I would very much like to switch to LEDs, therefore we consider Lumencor 
"SOLA-SM II - White LED source". An article refered to earlier on this 
list (doi: 10.7171/jbt.14-2502-001 
<http://dx.doi.org/10.7171%2Fjbt.14-2502-001>) described that light 
intensitiy of a SOLA is higher over the whole relevant than a "120 W 
metal Halide" lamp (Fig 1, 
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970759/figure/F1/), so this 
seemed to be a good choice.

I know however heard from two sources that I consider reliable that the 
SOLA has significantly less output in the excitation range of orange and 
near red dyes (maybe 540 to 600) than a EL6000 using a HXP R 120W metal 
halogenid lamp.

Therefore I would appreciate if you could share your thoughts on and 
experience with the SOLA, in particular with orange and near red dyes.

Thanks

Steffen


-- 
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

Marchioninistr. 27
D-81377 München
Germany