*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi everybody,

I need to a quantification in a z-stack I dont know which is the best way to do it?
I was try to do it with image J in 3D reconstruction but my problems is when 
imageJ and the other software generate the 3d reconstruction it make a average 
with the fluorescence to generate the 3D reconstruction, And the other thing is I 
dont know if in imagej is posible select an area in the photo a request the 
quantiication in all the slides, Because doing the quantification in each slide is 
really slow and complicate i think. mmm ImageJ has a special tools or plugin to 
do the quantification? Because I was tried using the tools to select area and then 
generate histogram or measure options.

Thanks 
Carlos