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There actually is a 2d deconvolution setup available for ImageJ.  
Check the plugins by Robert Dougherty.  I have used it with great 
success.  The trick is in deciding how to set up the PSF.

Joel


Date sent:      	Thu, 23 Mar 2006 01:58:33 -0500
Send reply to:  	Confocal Microscopy List <[log in to unmask]>
From:           	Ricardo Armisen <[log in to unmask]>
Subject:        	home made deconvolution setup
To:             	[log in to unmask]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hi all
> a question about deconvolution (again!!!!!)
> I am trying to put together a "home made" time lapse microscope (for
> gfp tag proteins and slow calcium signals) with deconvolution in mind,
> would something like this work?
> 
> -a IM 35 zeiss inverted microscope (the 160 length tube!!) with a good
> 63x/NA 1.3 oil objective (but no luck getting a water one so far) - a
> spot 2 (slider) ccd camera (diagnostic instruments) - a jena piezo
> device for the objective (any bet if the extra 11 mm will interfere
> with the optics?)..this is optional - a shutter for the mercury lamp
> and an optical excitation switch (DX-1000, Solameretech) - run by and
> old version of QED imaging software
> 
>  the deconvolution would be made afterwards image acquisition. I don't
>  plan
> to do any fancy 4D imaging, just one picture (one focal plane) over
> time, but most deconvolution free software (ImageJ and Image Track) I
> had found seem to be done for xyz stacks, I was able to trick the
> software supplying a psf made  of only one picture (not much of a
> psf...), but I am not sure if this is the rigth procedure, do you
> think it is posible to get good plain 2d deconvolution?  thanks....
> 
> Ricardo Armisen
> 
> SUNY at SB
> 
> U. de Chile

Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [log in to unmask]