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Are you using confocal or widefield, was that image a single plane or  
a brightest point projection? what is the label and mountant?

Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
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The box said "Requires WindowsXP or better", so I bought a Macintosh.
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On Feb 6, 2008, at 12:28 PM, Xinyu Zhao wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>
>
> Dear listers,
>
>  I was wondering if anybody could give me some advice on how to  
> count the
> objects in the image as attached.  They were cross sections of  
> inner segments
> of rods and the goal is to get the total number of them.
>
> It seemed a easy job at the beginning.  But after I started, I  
> realizee that it
> is tricky.  The intensity variation of the objects is big and the  
> boundaries of
> the cells are not clear.
>
> I am not much of an image processing person.  I was wondering if  
> anybody could
> give me some advice on how to proceed.
>
> Thank you very much.
>
> Xinyu Zhao
> Biomedical Imaging Core Lab
> B110 Richards Building
> School of Medicine
> University of Pennsylvania
> 37th and Spruce Street
> Philadelphia, PA 19104<#1978, ODA, 1,000um from ONH, 40X.tif>