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Greetings Mary,
    It is a common convention to express intensity values in terms of
Arbitrary Units (A.U.) on a scale which reflects the bit-depth of the
sensor used (in your case 8-bit; scale of 0-255 A.U.).  An alternative
is to normalize the intensity values to correspond to a scale of 0 to 1.
Regards,
Karl

Mary Ellen Pease wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi all,
>
>I have spent a couple hours "google"ing and reviewing manuals/pdfs
>provided by Zeiss without success to locate the following:
>
>What are the units for the emission intensity graphs (and tables) one
>can create when selecting an ROI within a  lambda stack?  When the graph
>data are not normalized, the Y axis for intensity appears to read from
>0-250 (with an 8 bit image - I've not taken any 12 bit yet), yet no unit
>is given.  Does this mean that it is reading brightness on a scale of
>0-256 like an 8 bit digital cameras would and if so, what is the proper
>technical label for this attribute?
>
>Thanks all.
>
>Mary Ellen Pease, M.S.
>Laboratory Manager
>Glaucoma Research Lab & Microscopy and Imaging Core Facility
>Wilmer Eye Institute, Johns Hopkins Hospital
>175 Woods Research
>600 N. Wolfe Street
>Baltimore, MD 21287
>410-955-3337 (phone/voicemail)
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--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
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Phone: 217.244.6292
Fax: 217.244.6219
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www.itg.uiuc.edu